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目的了解甘肃省嘉峪关市市售食品中食源性致病菌的污染状况。方法依据中国疾病预防控制中心营养与食品安全所制定的《食源性致病菌监测工作手册》和国家标准《食品卫生微生物学检验》(GB/T 4789)规定的检测程序和方法对样品进行金黄色葡萄球菌、阪崎肠杆菌、蜡样芽孢杆菌等9种食源性致病菌进行检测分离、鉴定。结果2011—2013年共采集嘉峪关市市售食品14类951份食品样本,检出食源性致病菌97份,阳性菌株103株,总检出率为10.83%;其中,2011年、2012年和2013年食源性致病菌检出率分别为27.27%、11.56%、4.06%,豆制品中食源性致病菌检出率最高,为26.67%;其次为餐饮食品、蛋与蛋制品及婴幼儿食品,检出率分别为21.33%、18.18%、12.17%。结论嘉峪关市市售食品食源性致病菌污染较为严重,主要集中在豆制品、餐饮食品、蛋与蛋制品、婴幼儿食品等;食源性致病菌以蜡样芽孢杆菌和金黄色葡萄球菌为主。
Objective To understand the status of foodborne pathogens in Jiayuguan City, Gansu Province. Methods Based on the testing procedures and methods of Food Safety Microbiology Test (GB / T 4789) and Food Safety Microbiological Test (GB / T 4789) established by Nutrition and Food Safety of China Center for Disease Control and Prevention, Staphylococcus aureus, Enterobacter sakazakii, Bacillus cereus and other 9 kinds of food-borne pathogenic bacteria were isolated and identified. Results A total of 951 food samples of 14 types of food were collected from Jiayuguan City in 2011-2013. 97 food-borne pathogens and 103 strains of positive bacteria were detected, with a total detection rate of 10.83%. Among them, 2011 and 2012 And the detection rate of food-borne pathogens in 2013 was 27.27%, 11.56% and 4.06% respectively. The highest detection rate of food-borne pathogens in soy products was 26.67%, followed by catering food, egg and egg products And infant food, the detection rate was 21.33%, 18.18%, 12.17%. Conclusion The food-borne pathogens in Jiayuguan City are more polluted, mainly in soy products, food and beverage, egg and egg products, infant food and so on. Food-borne pathogens are Bacillus cereus and golden yellow grape Cocci based.