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目的探讨IgE高亲和力受体MS4A2基因外显子及其侧翼区碱基序列单核苷酸多态性与哮喘气虚体质患儿相关性。方法选取58例气虚体质哮喘患儿作为哮喘组,50例健康体检儿童作为实验对照组。提取哮喘组和对照组外周血基因组DNA样本,应用PCR技术扩增MS4A2基因外显子及其侧翼区序列。扩增产物直接测序,确定碱基类型。结果通过测序结果分析,发现三个内含子多态(rs1441586,rs2847663,rs512555),一个外显子多态(rs569108)。rs569108位点AG基因型频率高于正常对照组(χ2=7.938,P=0.0189)。结论 rs569108位点AG型多态可能是导致气虚体质患儿哮喘的易感因素。
Objective To investigate the association between single nucleotide polymorphisms of exon and its flanking region of MS4A2 gene of IgE high affinity receptor and asthmatic children with asthenia asthenia. Methods Fifty-eight children with asthenia asthma were selected as asthma group and 50 healthy children as experimental control group. Genomic DNA samples from peripheral blood of asthmatic group and control group were extracted, and exon and its flanking regions of MS4A2 gene were amplified by PCR. The amplified product is sequenced directly to determine the base type. Results According to the sequencing results, three intronic polymorphisms (rs1441586, rs2847663, rs512555) and one exon polymorphism (rs569108) were found. The frequency of AG genotype in rs569108 locus was higher than that in normal controls (χ2 = 7.938, P = 0.0189). Conclusion AG polymorphism at rs569108 may be the predisposing factor to asthma in asthmatic children.