Labyrinth channels are widely adopted in emitter designs to regulate the water flow. The flow regime and the head loss of labyrinth channels have significant impacts on the hydraulic performance of emitters. In this study, the flow behavior of water passing through an emitter channel is observed using the micro particle image velocimetry (PIV), and the head loss during the flow is analyzed for an emitter with a triangular labyrinth channel. The results show that the flow regime is consistent with the classical theory of hydraulics goving straight channels, even when the cross-sectional area is very small (as small as 0.5 mm×0.5 mm). The critical Reynolds number from laminar to turbulent flows in a labyrinth channel is approximately in a range between 43 and 94. The local head loss factor decreases as the Reynolds number increases for labyrinth channels with smaller cross-sectional areas, such as 0.5 mm×0.5 mm and 1.0 mm×1.0 mm. The local head loss factor is not related to the Reynolds number and is only a function of the boundary conditions of the labyrinth channel when the Reynolds number exceeds approximately 1 000 (for cross-sectional areas of 1.5 mm×1.5 mm and 2.0 mm×2.0 mm). The ratio of the local head loss to the total head losstotal(hj/hf) first increases and then remains nearly constant as the Reynolds number increases in the labyrinth channel. The head loss in the labyrinth channel is almost equal to the local head loss, andtotal(hj/hf) is approximately 0.95 for cross-sectional areas of greater than 1.0 mm×1.0 mm. These results can be used for optimizing the design of emitter channels.