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Objective To detect the content of luteolin in CAULIS LONICERAE JAPONICAE.Methods HPLC was adopted.Chromatographic column was Agilent ZORBAX SB-C18 (4.6 mm × 250 mm,5 μm); mobile phase was anhydrous methanol0.3% phosphoric acid solution (47∶53,V/V); flow rate was 1 mL/min; column temperature was 30 ℃; and detection wavelength was 350 nm.Results The linear range of luteolin was between 0.5 and 2.5 μg/mL.Correlation coefficient was R=0.999 6; average recovery rate was 100.71%,and RSD was 1.9%.The average content of luteolin in three batches of CAULIS LONICERAE JAPONICAE was 24.05 μg/g.Conclusions This method was simple,rapid,reliable and could be used for the quality control of CAULIS LONICERAE JAPONICAE.
Objective To detect the content of luteolin in CAULIS LONICERAE JAPONICAE.Methods HPLC was applied. Chromatographic column was Agilent ZORBAX SB-C18 (4.6 mm × 250 mm, 5 μm); mobile phase was anhydrous methanol 0.3% phosphoric acid solution (47: 53, V / V); flow rate was 1 mL / min; column temperature was 30 ° C .; and detection wavelength was 350 nm. Results The linear range of luteolin was between 0.5 and 2.5 μg / mL. Correlation coefficient was R = 0.999 6 ; average recovery rate was 100.71% and RSD was 1.9%. The average content of luteolin in three batches of CAULIS LONICERAE JAPONICAE was 24.05 μg / g. Confclusions This method was simple, rapid, reliable and could be used for the quality control of CAULIS LONICERAE JAPONICAE.