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探讨了单次经口给予桑叶提取物对大鼠小肠微粒体的CYP3A活性的影响。方法:①大鼠小肠微粒体的制备:对Wistar系雄性大鼠(9周龄)口饲各种浓度的桑叶提取物混悬液,适时取出小肠按常规方法进行制备。②CYP3A活性:在NADPH生成系存在的条件下,上述微粒体(蛋白量300g)及硝苯地平(NFP10
The effects of a single oral administration of Mulberry extract on CYP3A activity in rat small intestinal microsomes were investigated. METHODS: 1 Preparation of rat small intestinal microsomes: Wistar male rats (9 weeks old) were orally fed with various concentrations of Mulberry leaf extract suspension, and the small intestine was timely removed and prepared according to conventional methods. 2CYP3A activity: In the presence of NADPH production system, the above-mentioned microsomes (protein amount 300 g) and nifedipine (NFP10)