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目的 观察慢性丙型肝炎患者外周血淋巴细胞对丙型肝炎病毒（ＨＣＶ） 抗原刺激的增殖反应．方法 外周血单个核细胞（ＰＢＭＣ） 与ＨＣＶ 抗原ｃ２２ 、ｃ３３ 、ｃ１００ － ３ 、ＮＳ５ 和植物血凝素（ＰＨＡ） 分别共同孵育，加入胸腺嘧啶核苷（３ ＨＴｄＲ） ，然后收集细胞于液闪仪测定每分钟脉冲数（ｃｐｍ） ．结果 根据对不同ＨＣＶ 抗原的淋巴细胞增殖反应发现，以ｃ２２免疫原性最强，ｃ１００ － ３ 次之：淋巴细胞激活与ＨＣＶ 基因型关系不大；健康对照和慢性乙型肝炎患者对各ＨＣＶ 抗原未能显示有效的淋巴细胞增殖反应；与健康对照比较，慢性丙型肝炎和乙型肝炎患者对ＰＨＡ 刺激的淋巴细胞增殖反应降低．结论 ＨＣＶ 抗原ｃ２２ 免疫原性最强，丙型肝炎患者对ＨＣＶ 抗原的淋巴细胞增殖反应系特异性；慢性丙型肝炎和乙型肝炎患者存在抑制的细胞免疫应答．
Objective To observe the proliferative response of peripheral blood lymphocytes to hepatitis C virus (HCV) antigen stimulation in patients with chronic hepatitis C virus. Methods Peripheral blood mononuclear cells (PBMCs) were incubated with HCV antigens c22, c33, c100 - 3, NS5 and phytohemagglutinin (PHA) respectively. Thymidine (3 HTdR) The flash meter measures the number of pulses per minute (cpm). Results According to the lymphocyte proliferation responses to different HCV antigens, c22 immunogenicity was the strongest, while c100 - 3 times: lymphocyte activation was not related to HCV genotype. In healthy control and chronic hepatitis B patients, Failed to show a potent lymphocyte proliferative response; patients with chronic hepatitis C and hepatitis B had a lower response to PHA-stimulated lymphocyte proliferation than healthy controls. Conclusion The HCV antigen c22 has the strongest immunogenicity. The hepatitis C virus is specific to the lymphocyte proliferation of HCV antigens. There is an inhibitory cellular immune response in chronic hepatitis C and hepatitis B patients.