【摘 要】
：
The optimization of nutrient levels for the production of recombinant hyperthermophilic esterase by E. coli was carried out with response surface methodology(RS
【机 构】
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Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education
论文部分内容阅读
The optimization of nutrient levels for the production of recombinant hyperthermophilic esterase by E. coli was carried out with response surface methodology(RSM) based on the central composite rotatable design(CCRD). A 24central composite rotatable design was used to study the combined effect of the nutritional constituents like yeast extract, peptone, mineral salt and trace metals. The P-value of the coefficient for the linear effect of peptone concentration was 0. 0081 and trace metals solution was less than 0. 0001, suggesting that these were the principal variables with significant effect on the hyperthermophilic esterase production. The predicted optimal hyperthermophilic esterase yield was 269. 17 U/mL, whereas an actual experimental value of 284. 58 U/mL was obtained.
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