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目的 :观察重复正加速度 (+Gz)暴露对大鼠心肌血管内皮细胞超微结构的影响及其暴露后细胞间粘附分子 1(ICAM 1)表达的变化情况 ,进一步探讨高 +Gz暴露致心肌损伤的机理。方法 :30只雄性Wistar大鼠随机分成 3组 (n =10 ) ,其中对照组不受 +Gz作用 ;正加速度组分为 +1Gz组 :受 +1Gz作用 ,+10Gz组 :重复 +10Gz暴露 (+10Gz 30s,5counts/d ,3d/w ,3w)。于末次 +Gz作用后次日同时处死大鼠 ,速取左室心肌 ,常规透射电镜制样、观察。另取左室心肌制成石蜡切片 ,行免疫组化检测。结果 :重复 +10Gz作用后 ,心肌间质弥漫水肿 ,小血管内皮细胞肿胀 ,吞饮泡增多 ,血管内皮细胞ICAM 1含量也明显增加 (P <0 .0 5 ) ,而 +1Gz组心肌血管内皮结构及其I CAM 1含量与对照组之间无明显差别。结论 :重复 +Gz暴露后 ,大鼠心肌血管内皮细胞可出现明显损伤 ,其I CAM 1表达增多 ,提示粘附分子诱导的炎症反应参与了高 +Gz应激导致的心肌损伤
OBJECTIVE: To observe the effects of repeated positive acceleration (+ Gz) exposure on the ultrastructure of myocardial vascular endothelial cells in rats and the changes of ICAM-1 expression after exposure, and further explore the effects of + Gz exposure on myocardium Damage mechanism. Methods: Thirty male Wistar rats were randomly divided into three groups (n = 10), in which the control group was not exposed to + Gz; the positive acceleration component was + 1Gz group: +10 Gz group +10 Gz group + 10Gz 30s, 5 counts / d, 3d / w, 3w). Rats were killed at the same time the next day after the last + Gz treatment, and the left ventricular myocardium was taken as fast as possible. The samples were observed by transmission electron microscopy. Another left ventricular myocardial paraffin section, immunohistochemistry. Results: After repeated 10Gz administration, cardiac interstitial diffuse edema, swelling of small blood vessel endothelium, increase of swallowed vomit and ICAM 1 level in vascular endothelial cells were also significantly increased (P <0.05), while in + 1Gz group, There was no significant difference in the structure and I CAM 1 content between the two groups. CONCLUSION: After repeated + Gz exposure, myocardial vascular endothelial cells of rats show obvious damage, and the expression of I CAM 1 is increased, suggesting that the adhesion molecule-induced inflammatory reaction is involved in myocardial injury caused by high + Gz stress