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目的探讨AIDS(艾滋病)合并肺结核患者痰液两种方法培养及其耐药情况。方法采集患者痰液分别采用BacT/ALERT3D系统和罗氏法进行结核分枝杆菌的培养及药物敏感性测定。结果 341例AIDS合并肺结核患者痰液两种方法检测,共129例培养阳性,总阳性率39.81%,3D法培养污染率为2.6%,罗氏法培养污染率为2.3%。129株菌株药敏测定结果有40株出现耐药,耐药率为31.01%。结论 3D法在AIDS合并肺结核患者痰液培养方面较罗氏法阳性率高,两种方法相互结合更能提高培养效率。AIDS合并肺结核患者痰液结核菌培养和药敏实验可为临床诊断和指导用药提供重要依据。
Objective To investigate two methods of culturing sputum in patients with AIDS (tuberculosis) and tuberculosis (TB) and their drug resistance. Methods The sputum samples of patients were collected and analyzed by BacT / ALERT3D system and Roche method respectively for the detection of Mycobacterium tuberculosis and drug susceptibility. Results A total of 341 sputum samples were collected from 341 patients with AIDS complicated with pulmonary tuberculosis. A total of 129 samples were positive for culture, with a total positive rate of 39.81%. The contamination rate was 2.6% in 3D method and 2.3% in Roche method. Out of 129 strains, 40 strains showed resistance, with a resistance rate of 31.01%. Conclusion The 3D method is more effective than Roche method in the sputum culture of AIDS patients with pulmonary tuberculosis. The combination of the two methods can improve the culture efficiency. Mycobacterium tuberculosis culture and susceptibility testing of sputum in AIDS patients with pulmonary tuberculosis may provide an important basis for clinical diagnosis and guidance of medication.