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用CO2激光(20.1 mW/mm2)辐照小麦种子3 min,待其长至12天时,用质量浓度10%聚乙二醇6000(PEG6000)胁迫其幼苗,并通过添加NO的供体硝普钠(SNP)和血红蛋白(Hemoglobin,Hb;NO清除剂),研究NO在CO2激光预处理提高小麦耐旱性中的作用。结果表明,外源NO和激光预处理可使干旱胁迫的小麦幼苗丙二醛(MDA)含量显著降低(p<0.05),而超氧化物歧化酶(SOD),过氧化物酶(POD),过氧化氢酶(CAT)活性,叶绿素a,叶绿素b含量和根干重却显著增加(p<0.05)。而经过适当剂量激光辐照干旱胁迫小麦幼苗再加外源血红蛋白(Hb,NO清除剂)处理则没有这种作用。
The wheat seeds were irradiated with CO2 laser (20.1 mW / mm2) for 3 min. When the seeds were grown to 12 days, their seedlings were inoculated with 10% PEG6000 and the concentration of sodium nitroprusside (SNP) and hemoglobin (Hemoglobin, Hb; NO scavenger) to study the role of NO in CO2 laser pretreatment to improve the drought tolerance of wheat. The results showed that exogenous NO and laser pretreatment significantly decreased MDA content in wheat seedlings under drought stress (p <0.05), but SOD, POD, Catalase (CAT) activity, chlorophyll a, chlorophyll b and root dry weight increased significantly (p <0.05). However, irradiation of drought-stressed wheat seedlings with appropriate dose of laser irradiation plus exogenous hemoglobin (Hb, NO scavenger) did not.