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目的探讨己酮可可碱和苯那普利联用对阿霉素肾病大鼠细胞因子及尿蛋白的影响。方法 80只雄性SD大鼠,分为对照组、模型组、己酮可可碱组、苯那普利组及联合组。对模型组和三治疗组给予阿霉素(5 mg/kg)尾静脉注射,建立肾病综合征模型。自注射阿霉素后,己酮可可碱组和苯那普利组分别给予己酮可可碱50 mg/(kg.d)、苯那普利6 mg/(kg.d)、联合组给予己酮可可碱25 mg/(kg.d)联合苯那普利3 mg/(kg.d)灌胃,共6周。分别于给药2周、4周、6周抽血测肿瘤坏死因子-α、白介素-6及放入代谢笼留24 h尿测蛋白定量。结果肿瘤坏死因子-α、白细胞介素-6在己酮可可碱组、苯那普利组和联合组均较模型组明显降低(P均<0.01),而联合组又低于己酮可可碱组和苯那普利组(P均<0.05)。结论己酮可可碱和苯那普利联合应用能进一步降低肿瘤坏死因子-α和白介素-6的表达,减轻炎症反应,联合治疗疗效优于单独应用。
Objective To investigate the effects of pentoxifylline and benazepril on cytokines and urinary protein in adriamycin nephropathy rats. Methods Eighty male SD rats were divided into control group, model group, pentoxifylline group, benazepril group and combination group. The model group and three treatment groups were given doxorubicin (5 mg / kg) intravenous injection to establish nephrotic syndrome model. After injection of doxorubicin, pentoxifylline and benazepril groups were given pentoxifylline 50 mg / (kg · d) and benazepril 6 mg / (kg · d), respectively, and the combination group was given Keto-theobromine 25 mg / (kg.d) combined benazepril 3 mg / (kg.d) orally for 6 weeks. The levels of tumor necrosis factor-α, interleukin-6 and urine protein in the metabolic cage for 24 h were determined by blood sampling at 2 weeks, 4 weeks and 6 weeks respectively. Results Compared with model group, the levels of TNF-α and IL-6 in pentoxifylline group and benazepril group were lower than those in model group (all P <0.01), while those in combination group were lower than those in pentoxifylline Group and benazepril group (all P <0.05). Conclusion Combination of pentoxifylline and benazepril can further reduce the expression of tumor necrosis factor-α and interleukin-6 and reduce the inflammatory reaction, and the combined treatment is better than single application.