The inclusion interaction between three types of methylated-β-cyclodextrins(Me-β-CDs)and albendazole(ABZ)was studied by spectrofluorimetry.The result showed that Me-β-CDs reacted with ABZ to form an inclusion complex,1:1 stoichiometry for Me-β-CDs-ABZ complex was established and its association constant have been determined from fluorescence data by Benesi- Hildebrand’s method(double reciprocal plots).It was noted that 2,6-DM-β-CD exhibited stronger binding ability than other Me-β- CDs.Based on the significant enhancement of fluorescence intensity of inclusion complex,a simple and highly sensitive fluorimetric method is proposed for the determination of ABZ in the presence of 2,6-DM-β-CD.The proposed method was successfully applied to the determination of ABZ in tablets and human urine. The inclusion interaction between three types of methylated-β-cyclodextrins (Me-β-CDs) and albendazole (ABZ) was studied by spectrofluorimetry. The result showed that Me-β-CDs reacted with ABZ to form an inclusion complex, 1: 1 stoichiometry for Me-β-CDs-ABZ complex was established and its association constant have been determined from fluorescence data by Benesi-Hildebrand’s method (double reciprocal plots) .It was noted that 2,6-DM-β-CD was stronger binding ability than other Me-β-CDs. Based on the significant enhancement of fluorescence intensity of inclusion complex, a simple and highly sensitive fluorimetric method is proposed for the determination of ABZ in the presence of 2,6-DM-β-CD. The proposed method was successfully applied to the determination of ABZ in tablets and human urine.