凝胶色谱和计算机联机进行自动数据采集的过程完成以后,采集的数据并不能立即投入谱图的计算过程,因为仪器及计算机采集系统在工作中受到温度、外界干扰、检测器信号飘移、随机噪声等因素的影响。采集的数据是包括以上这些因素的整个系统的表观数据,要得到真实的凝胶色谱图,一定要对这些数据进行予处理。一、谱图奇异点的修正由于谱图变化具有连续性,因此,对于由外界干扰而产生的奇异点可以用一阶差分法来检拾,其数学表达式为:H测’=从一1十(从一1一从一二)(1)式中 H娜,从一1,风一二分别表示第乞点的预测值及第葱一 Gel chromatography and computer online automatic data acquisition process is completed, the collected data can not be immediately put into the spectrum calculation process, because the instrument and computer acquisition system at work by temperature, external interference, the detector signal drift, random noise And other factors. The data collected is the overall system of apparent data that includes these factors. To get a true gel chromatogram, these data must be processed. First, the correction of the singular point of the spectrum As the spectrum changes with continuity, so for the singularities generated by external interference can be used first-order difference method to pick up, the mathematical expression is: Ten (from one to one from one to two) (1) where H Na, from a 1, the wind one two, respectively, said the predicted value of beggar point and the first one