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参照Ｂ．Ｊ．Ｓｍｉｒｂ（１９８４）介绍的蛋白质ＳＤＳ—ＰＡＧＥ方法，调整了凝胶浓度、交联度及催化剂用量，提出了完善的样品制备程序，并研究了温度对凝胶配比及电泳时间的影响。在大量小麦整粒ＳＤＳ—ＰＡＧＥ实验的基础上，又对半粒无胚籽粒电泳进行了细致的研究，摸荣出了一套适合于小麦整粒与半粒的高分子量（ＨＭＷ）麦谷蛋白亚基ＳＤＳ—ＰＡＧＥ方法。通过对５０７１份整粒种质资源和１６００余份半粒杂交后代的分析，结果表明，该法成功率高，结果准确，具有操作简便、快速、分辨率好，制成干板不扩散易保存等优点。认为是一套适合我国仪器设备和药剂等实验条件的ＨＭＷ谷蛋白电泳方法。是品质鉴定和后代品质筛选的可靠手段。
Refer to B. J. Smirb (1984) introduced the protein SDS-PAGE method, adjusting the gel concentration, crosslinking degree and the amount of catalyst, put forward a perfect sample preparation procedure, and studied the temperature on the gel ratio and electrophoresis time. Based on a large number of whole wheat SDS-PAGE experiments, the semi-particle asexual seed electrophoresis was studied in detail and a set of high molecular weight HMW glutenin subunits Based SDS-PAGE method. The results showed that the method has high success rate and accurate result, which is easy to operate, fast and has good resolution and easy to store without proliferation. Etc. It is considered as a set of HMW gluten electrophoresis method suitable for the experimental conditions of China’s equipment and pharmaceuticals. It is a reliable method for quality identification and quality screening of offspring.