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实时荧光定量PCR技术以其特异性强、灵敏度高、重复性好、自动化程度高、检测速度快等优点在动物疫病的监测、检测和诊断中发挥巨大的作用,但是影响实时荧光定量PCR技术的因素却不容忽视。试验就实时荧光定量PCR技术的影响因素及相应对策进行综合分析,以提高动物疫病监测、检测和诊断的水平。1样品处理因素1.1样品的采集应按照活体动物样品的采集要求采集猪扁桃体、家禽咽肛拭子、牛羊食道-咽部分泌物(O-P液)、
Real-time fluorescence quantitative PCR technology plays a great role in the monitoring, detection and diagnosis of animal epidemics because of its strong specificity, high sensitivity, good repeatability, high degree of automation and fast detection speed. However, the real-time fluorescent quantitative PCR Factors can not be ignored. The experiment carried on the comprehensive analysis to the influencing factor and the corresponding countermeasure of the real-time fluorescence quantitative PCR technology, in order to raise the level which the animal epidemic disease monitor, the examination and the diagnosis. 1 sample processing factors 1.1 sample collection should be collected in accordance with the requirements of live animal samples pig tonsil, poultry pharyngeal swabs, cattle and sheep esophageal - pharyngeal secretions (O-P liquid)