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目的:探讨Ras超家族的小G蛋白Ran GTPase在胰腺癌组织及细胞中的表达情况,及其对胰腺癌细胞PANC-1增殖和凋亡的影响。方法:选取胰腺癌石蜡标本及相应正常组织标本各27例进行免疫组织化学染色。用LipofectamineTM2000转染Ran干扰RNA(small interference RNA,siRNA)进入胰腺癌细胞系PANC-1干扰其表达,之后利用Western blot观察Ran的表达情况。运用MTT及流式细胞术分别检测各实验组细胞的增殖和凋亡情况。结果:免疫组化染色显示在胰腺癌组织中Ran的表达阳性率及得分均明显高于癌旁正常组织(P<0.05)。将干扰Ran siRNA转染进入细胞系PANC-1中,利用Western blot发现Ran的表达明显下调。并且通过MTT实验发现在胰腺癌细胞系PANC-1中下调Ran表达能明显抑制该细胞生长(P<0.05),并使PANC-1细胞凋亡显著增多(P<0.05)。结论:小G蛋白Ran GTPase在胰腺癌组织及细胞系中高表达,且Ran可以促进胰腺癌细胞PANC-1的增殖,抑制其凋亡。
Objective: To investigate the expression of small G protein Ran GTPase in pancreatic cancer tissues and cells and its effect on the proliferation and apoptosis of pancreatic cancer cell line PANC-1. Methods: Immunohistochemical staining was performed on 27 cases of pancreatic cancer paraffin specimens and corresponding normal tissue specimens. LipofectamineTM2000 was used to transfect Ran interference RNA (siRNA) into pancreatic cancer cell line PANC-1 to interfere its expression. Then the expression of Ran was detected by Western blot. MTT and flow cytometry were used to detect the cell proliferation and apoptosis in each experimental group. Results: Immunohistochemical staining showed that the positive expression rate and the score of Ran in pancreatic cancer tissues were significantly higher than those in the adjacent normal tissues (P <0.05). The interference Ran Ran siRNA transfection into the cell line PANC-1, using Western blot found that the expression of Ran was significantly down-regulated. MTT assay showed that the down-regulation of Ran expression in pancreatic cancer cell line PANC-1 significantly inhibited the cell growth (P <0.05) and significantly increased the apoptosis of PANC-1 cells (P <0.05). Conclusion: The small G protein Ran GTPase is highly expressed in pancreatic cancer tissues and cell lines, and Ran can promote the proliferation and inhibit the apoptosis of pancreatic cancer PANC-1 cells.