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目的 建立一种标准化的大鼠视神经不同程度部分损伤动物模型 ,对致伤强度和损伤程度以及它们之间的关系进行量化分析。 方法 用压力为 1 4 8.0g的反向镊夹持大鼠视神经3,6 ,1 2 ,30 ,6 0s,或用压力为 32 .4g的微型动脉夹夹持大鼠视神经 5 ,1 0 ,1 5s,建立不同程度视神经损伤的动物模型 ,用经颅荧光金逆行标记视网膜节细胞 (retinalganglioncell,RGC)的方法 ,观察损伤后 1个月RGC的变化 ,以RGC数目的变化衡量损伤程度 ,并用坚牢蓝经心脏灌注的方法 ,评价该致伤模型中眼部血供的变化。 结果 用致伤冲量和平均冲量能全面地反映致伤强度 ,荧光金逆行标记RGC计数是衡量损伤程度的良好指标。微型动脉夹夹持大鼠视神经 1 5s与反向镊夹持3s的平均致伤冲量相等 ,引起的RGC数目的变化也相似。随着致伤强度的增加 ,RGC数目减少 ,RGC的数目与致伤冲量之间呈幂函数曲线关系。短时间的视神经夹持不会引起视网膜的缺血损伤。 结论 利用反向镊夹持的方法可以很简便地建立一种易于标准化的视神经部分损伤模型 ,该模型可以用冲量、平均冲量和RGC计数的方法来评价。
OBJECTIVE: To establish a standardized animal model of partial lesion of optic nerve in rats and quantitatively analyze the degree of injury, the degree of injury and the relationship between them. Methods The rat optic nerve was grasped by reverse tweezers at a pressure of 14.08g for 3, 6, 12, 30, 60s, or with a clamping force of 32.4g. The animal models of optic nerve injury were established at different time points. The retinal ganglion cells (RGCs) were retrogradely labeled with transcranial fluorescent gold to observe the changes of RGC at 1 month after injury. Fasting was perfused via cardiac perfusion to assess changes in ocular blood supply in this model of injury. Results Injury impulse and average impulse can fully reflect the injury intensity, fluorescent gold retrograde labeling RGC count is a good indicator of the degree of injury. The average injury impulse of the microtheremous clip holding the optic nerve of rat for 15s and the reverse tweezer holding for 3s were the same, and the changes of the number of RGCs were also similar. With the increase of wounding intensity, the number of RGCs decreased and the number of RGCs showed a power function curve relationship with the injury impulse. Short-term optic nerve grafts do not cause ischemic damage to the retina. CONCLUSIONS: An easy-to-standard model of optic nerve partial damage can be easily and conveniently established using reverse tweezers, which can be evaluated by impulse, mean impulse and RGC counting.