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①目的 探讨人乳头瘤病毒 11,16型 (HPV11,16 )转化基因E6DNA及HPV16E6mRNA与喉疾病严重程度的关系。②方法 分别用竞争性聚合酶链反应 (CPCR)和半定量RT PCR技术检测喉乳头状瘤、喉鳞癌组织中HPV11,16E6DNA和HPV16E6mRNA的含量。③结果 喉鳞癌组织中HPV11,16E6DNA和HPV16E6mRNA的含量均高于喉乳头状瘤组织 ,差异有显著性 (t=3.74 5 ,2 .776 ,2 .75 9,P <0 .0 1,0 .0 5 ) ;高、中、低分化喉鳞癌组织中HPV11,16E6DNA含量无显著性差异 (F =0 .335 ,0 .2 34,P >0 .0 5 ) ,但低分化喉鳞癌组织中HPV16E6mRNA的表达水平显著高于高分化喉鳞癌 (F =4 .2 19,q =4 .10 7,P <0 .0 5 )。 ④结论 HPV11,16E6DNA和HPV16E6mRNA的含量有随病理程度加重而增高的趋势 ,从分子水平探讨转化基因E6的致癌性 ,有助于阐明HPV与喉癌发生发展的关系。
①Objective To investigate the relationship between HPV16E6 mRNA and HPV16, HPV16, HPV11, HPV16, HPV6, HPV6, and Severity of laryngeal diseases. Methods The contents of HPV11, HPV-16, HPV16E6 and HPV16E6 in laryngeal papilloma and laryngeal squamous cell carcinoma were detected by competitive polymerase chain reaction (CPCR) and semi-quantitative RT-PCR. Results The contents of HPV11, 16E6 DNA and HPV16E6 mRNA in laryngeal squamous cell carcinoma tissues were significantly higher than those in laryngeal papilloma tissues (t = 3.74 5, 2.776, 2.759, P <0.01 .0 5). There was no significant difference in HPV11, HPV16E6 DNA between high, moderate and poorly differentiated laryngeal squamous cell carcinoma (F = 0.335, 0.234, P> 0.05), but poorly differentiated laryngeal squamous cell carcinoma The expression of HPV16E6 mRNA in tissues was significantly higher than that in well differentiated laryngeal squamous cell carcinoma (F = 4.19, q = 4.107, P <0.05). Conclusion The levels of HPV11, HPV16E6 and HPV16E6 mRNA increased with the increase of pathology. To explore the carcinogenicity of transgene E6 from the molecular level may help to clarify the relationship between HPV and laryngeal carcinoma.