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以不同基因型的油菜种子为实验材料 ,以下胚轴为外植体 ,采用不同配比激素组合的培养基诱导芽苗分化。通过对芽苗分化率的方差分析明确了组织培养中激素对植株再生的影响大于基因型 ,且激素与基因型之间存在着显著的互作效应。MS+6-BA2～ 4 mg/ L+NAA0 .6mg/ L为诱导芽苗分化的最佳培养基
The rapeseed with different genotypes was used as experimental material, the hypocotyls were used as explants, and the shoots were induced to differentiate by using different combinations of hormones. Through the analysis of variance of shoot differentiation rate, the effect of hormone on plant regeneration in tissue culture was more obvious than genotype, and there was significant interaction between hormone and genotype. MS + 6-BA2 ~ 4 mg / L + NAA0 .6 mg / L is the best medium for inducing sprouting