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目的:研究鸡矢藤药材的UPLC指纹图谱。方法:采用Aglient ZORBAX SB-C_(18)(2.1mm×50mm,1.8μm)色谱柱,流动相为乙腈-0.1%甲酸水溶液梯度洗脱,流速为0.25mL/min,检测波长为250nm,柱温为30℃,进样量为1μL。并对测得的指纹图谱进行相似度评价、主成分分析和聚类分析。结果:21批不同样品共确立了9个共有峰,建立了鸡矢藤药材的UPLC对照指纹图谱。通过聚类分析,将鸡矢藤药材分为2类。通过对9个共有峰主成分分析,提取了3个主成分。结论:建立的方法快速简便,精密度、重复性、稳定性良好,可用于鸡矢藤药材的品质分析。
Objective: To study UPLC fingerprinting of Fructus Chinensis. Methods: Aglient ZORBAX SB-C 18 column (2.1 mm × 50 mm, 1.8 μm) was used. The mobile phase consisted of a gradient of acetonitrile-0.1% formic acid in water with a flow rate of 0.25 mL / min and a detection wavelength of 250 nm. For 30 ℃, the injection volume is 1μL. Similarity evaluation, principal component analysis and clustering analysis of the measured fingerprints were carried out. Results: Nine common peaks were identified in 21 batches of different samples, and UPLC fingerprinting was established. By cluster analysis, the chicken rotten vine is divided into two categories. By analyzing the principal components of nine common peaks, three principal components were extracted. Conclusion: The established method is rapid, simple, accurate, reproducible and stable, and can be used for the quality analysis of Radix et Caulis.