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检测非小细胞肺癌(non-small cell lung cancer,NSCLC)患者FUS2SNP s(767A/T)的多态性,并探讨其相关的临床意义。方法:收集2010-01-2011-12治疗的NSCLC患者84例,另选取体检科的健康体检者20名作为正常对照。SNPs多态性测定使用Real-Time PCR Taqman分析。结果:NSCLC患者A/A基因型频率为30.9%,A/T为35.7%,T/T为33.3%,对照组中分别为20.0%、55.0%和25.0%,A/A和T/T基因型在两组之间差异无统计学意义,P>0.05;A/T基因型在NSCLC组为55.0%,对照组为35.7%,两组之间具有统计学差异,χ2=4.62,P<0.05;NSCLC患者男和女性A/T基因型频率为37.0%和34.2%,差异无统计学意义,χ2=0.64,P>0.05;吸烟和不吸烟A/T基因型频率为38.9%和33.3%,差异无统计学意义,χ2=0.89,P>0.05;而在组织学分化程度上,高分化和中低分化A/T基因型频率为47.1%和28.0%,差异有统计学意义,χ2=3.89,P<0.05;淋巴结转移无和有A/T基因型频率为50.0%和25.0%,差异有统计学意义,χ2=4.35,P<0.05;临床病理分期Ⅰ、Ⅱ期和Ⅲ、Ⅳ期A/T基因型频率为45.5%和25.0%,差异有统计学意义,χ2=3.66,P<0.05。结论:FUS2SNP s(767A/T)基因型与NSCLC的易感性密切相关,可在NSCLC的早期诊断诊断、临床分级以及预后评估等方面起到一定的作用。
To detect the polymorphism of FUS2SNP s(767A/T) in patients with non-small cell lung cancer (NSCLC), and to explore its related clinical significance. Methods: 84 patients with NSCLC treated from 2010 to 2011-12 were collected, and 20 healthy examinees from the physical examination department were selected as normal controls. SNPs polymorphisms were measured using Real-Time PCR Taqman analysis. Results: The frequency of A/A genotype in NSCLC patients was 30.9%, A/T was 35.7%, T/T was 33.3%, in the control group were 20.0%, 55.0%, and 25.0%, respectively. A/A and T/T genes There was no significant difference between the two groups, P>0.05; A/T genotype was 55.0% in NSCLC group and 35.7% in control group. There was statistical difference between the two groups, χ2=4.62, P<0.05 The frequency of male and female A/T genotypes in NSCLC patients was 37.0% and 34.2%, and the difference was not statistically significant (χ2=0.64, P>0.05). The frequency of smoking and non-smoking A/T genotypes was 38.9% and 33.3%. The difference was not statistically significant (χ2=0.89, P>0.05). On the degree of histological differentiation, the frequencies of well-differentiated and moderately-differentiated A/T genotypes were 47.1% and 28.0%, the difference was statistically significant, χ2=3.89. ,P<0.05; No and no A/T genotype frequencies of lymph node metastasis were 50.0% and 25.0%, the difference was statistically significant, χ2=4.35, P<0.05; clinical pathological stages I, II and III, IV A The frequency of the /T genotype was 45.5% and 25.0%, and the difference was statistically significant (χ2=3.66, P<0.05). Conclusion: The FUS2SNP s(767A/T) genotype is closely related to the susceptibility of NSCLC, which may play a role in the early diagnosis, diagnosis, clinical classification and prognosis of NSCLC.