论文部分内容阅读
目的 探索用TNF α基因修饰联合冷冻法制备更高效能的抗肝癌疫苗的可能性。方法 用TNF α基因修饰和冷冻处理的H2 2细胞制成疫苗A ,用TNF α基因修饰未经冷冻处理的H2 2细胞制成苗B ;另外 ,用未经TNF α基因修饰行冷冻处理和未行冷冻处理的H 2 2细胞 ,分别制备疫苗C和D。比较这 4种疫苗的抗小鼠接种性肝癌的作用。结果 用TNF α基因修饰和冷冻处理H2 2细胞制成的疫苗 ,其降低接种性肝癌发生率的作用 ,提高脾淋巴细胞IL 2和TNF诱生水平及脾脏CTL对H2 2细胞的杀伤活性的作用 ,均明显优于其他 3种疫苗。结论 冷冻处理TNF α基因修饰的H2 2细胞 ,可提高该细胞的免疫原性 ,从而可提高疫苗的抗小鼠接种性肝癌的效能
OBJECTIVE: To explore the possibility of using TNFα modified gene in combination with freezing to prepare a more effective anti-HCC vaccine. Methods Vaccine A was prepared from H2 2 cells modified and frozen with TNFα gene and seedlings B were prepared by modifying the untransfected H2 2 cells with TNFα gene. In addition, cryoprotectants without TNF α gene modification H 2 2 cells were cryogenically treated to prepare vaccines C and D, respectively. Comparison of the four vaccines against mouse inoculation of the role of liver cancer. Results Vaccination with TNFα gene and cryogenic treatment of H2 2 cells decreased the incidence of inoculated HCC, increased the levels of IL 2 and TNF production in splenic lymphocytes and the cytotoxicity of spleen CTL to H 2 2 cells , Were significantly better than the other three kinds of vaccines. Conclusion Cryopreservation of TNFα gene modified H2 2 cells can enhance the immunogenicity of the cells and thus enhance the efficacy of the vaccine against mouse hepatocellular carcinoma