本文叙述用马来丝虫和彭亨丝虫感染期幼虫在体外培养至第4期和第5期获得了成功。作者所用的体外培养系统能使幼虫存活7周以上并被用作收集这些幼虫分泌、排泄和脱皮抗原的来源。由压碎东乡伊蚊所得的感染期幼虫用加有青霉素(100μ/ml)和链霉素(100μg/ml)的RPMI_(1640)。培养液洗涤3次,然后将其放入30ml容量的有螺旋帽的组织培养瓶中,添加10%灭活人AB血清和LLC-MK_2恒河猴肾细胞作为饲养层的RPMI-1640液进行培养。每瓶盛培养液5ml放入40至50条感染期幼虫并在“空气”中于37℃孵育。在无菌条件下隔天更换培养液一次,使LL-MK_2细胞系长满瓶底和瓶侧。在培养后期,培养液的pH This article describes the successful use of larvae infecting larvae of Malayian and Paenibacillus filarias in stages 4 and 5 in vitro. The in vitro culture system used by the authors enables larvae to survive for more than seven weeks and is used as a source for collecting the secretion, excretion and peeling antigens of these larvae. Infected larvae obtained from the crushing of Ae. Albopictus were treated with RPMI 1640 supplemented with penicillin (100 μ / ml) and streptomycin (100 μg / ml). The culture broth was washed three times and then placed in a 30 ml capacity screw-cap tissue culture flask and cultured with RPMI-1640 solution supplemented with 10% inactivated human AB serum and LLC-MK_2 rhesus kidney cells as a feeder layer . Place 5 ml of each flask containing 40 to 50 larvae of infection and incubate in “air” at 37 ° C. The culture medium was changed every other day under aseptic conditions to allow the LL-MK_2 cell line to grow over the bottom of the bottle and the bottle side. In the later stage of culture, the pH of the culture broth