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目的 研究氟在大鼠海马中的蓄积及其对胆碱酯酶活力的影响。方法 用氟化钠对大鼠进行亚慢性染毒 ,测定大鼠海马中氟浓度及胆碱酯酶活力。结果 大鼠海马内氟浓度与接触剂量呈正相关 ,高剂量组、低剂量组 [(1 3 .0 3± 1 .79)、(9.83± 0 .92 ) μg/g]与对照组 [(8.2 7± 1 .1 1 ) μg/g]比较或两剂量组间比较 ,差异均有显著性 (P <0 .0 1 ) ;乙酰胆碱酯酶 (AChE)活力高剂量组、低剂量组分别为 (0 .1 1 1± 0 .0 31 )、(0 .1 4 3± 0 .0 2 5) μmol/mg ,与对照组 (0 .1 83± 0 .0 2 7) μmol/mg比较或两剂量组间比较 ,差异均有显著性 (P <0 .0 1 ) ,且与海马内氟浓度呈负相关 (r=- 0 .70 0 ,P <0 .0 1 ) ;丁酰胆碱酯酶(TChE)活力在高剂量组 [(0 .0 4 1± 0 .0 1 0 ) μmol/mg]与对照组 [(0 .0 67± 0 .0 2 5) μmol/mg]间比较 ,差异有显著性 (P <0 .0 5) ,与海马内氟浓度的负相关关系不明显 (r =- 0 .31 7,P =0 .0 94)。结论 氟可透过血 脑屏障在大鼠海马内蓄积 ,进而抑制胆碱酯酶活力
Objective To investigate the accumulation of fluoride in rat hippocampus and its effect on the activity of cholinesterase. Methods Rats were sub-chronically exposed to sodium fluoride. The concentrations of fluoride and cholinesterase in hippocampus of rats were measured. Results The concentration of fluoride in the hippocampus of rats was positively correlated with the exposure dose. Compared with the control group [(8.33 ± 1.79), (9.83 ± 0.92) μg / g] 7 ± 1. 1 1) μg / g], or between the two dose groups, the difference was significant (P <0.01); AChE activity of high dose group and low dose group were ( 0 .1 1 1 ± 0 .0 31), (0 .143 ± 0 .025) μmol / mg compared to the control (0 .1 83 ± 0 .027) μmol / mg or both The difference between the two groups was significant (P <0.01), and negatively correlated with the fluoride concentration in hippocampus (r = - 0.70 0, P <0.01). Butyrylcholinesterase Compared with the control group [(0.67 ± 0.255 μmol / mg)], the enzyme (TChE) activity in the high dose group [(0.041 ± 0.010) μmol / mg] The difference was significant (P <0.05), but negatively correlated with fluoride concentration in hippocampus (r = -0.331, P = 0.094). Conclusion Fluorine can accumulate in the rat hippocampus through the blood-brain barrier, thereby inhibiting the activity of cholinesterase