重组大肠杆菌热稳定性过氧化氢酶的纯化及性质研究

来源 :微生物学报 | 被引量 : 0次 | 上传用户：liongliong579

【摘要】

：

将产热稳定性过氧化氢酶的重组大肠杆菌培养后菌体破碎得到的粗酶液经热处理、硫酸铵分级沉淀、DEAE-Sephadex A-50离子交换层析、HiPrep 16/10 Phenyl疏水作用层析、Su-Perd

【作者】

：

王凡强王正祥邵蔚蓝刘吉泉徐成勇诸葛健

【机构】

：

江南大学生物工程学院工业生物技术教育部重点实验室

【出处】

：

微生物学报

【发表日期】

：

2004年期

【关键词】

：

重组大肠杆菌过氧化氢酶纯化性质

下载到本地 , 更方便阅读

下载此文赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

将产热稳定性过氧化氢酶的重组大肠杆菌培养后菌体破碎得到的粗酶液经热处理、硫酸铵分级沉淀、DEAE-Sephadex A-50离子交换层析、HiPrep 16/10 Phenyl疏水作用层析、Su-Perdex200 HR 10/30凝胶层析提纯后得到电泳纯的酶,比酶活达到15629U/mg.此酶的最适温度为70℃,最适pH7.0,在60℃保温60min酶活力基本不变,在PH3～8的范围内比较稳定.此酶的Km和Vmax 分别为7.75mmol/L和27.8mnol@[email protected]/L的Zn2+、Ba2+、Mn2+可使该酶完全失活,KCN、NaN3、Na2S2O4、巯基乙醇对酶活力有抑制作用,50mmol/L的EDTA不影响酶活性.

其他文献

Lymphogranuloma venereum caused by Chlamydia trachomatis serovar L3: a case report

Lymphogranuloma venereum (LGV) is a systemic sexually transmitted disease caused by Chlamydia trachomatis (C. trachomatis) L1, L2 and L3, the organism gains ent

期刊

lymphogranuloma venereumChlamydia trachomatisserovar

Safety and efficacy of oral HD-03/ES given for six months in patients with chronic hepatitis B virus

AIM: To investigate the safety and efficacy of the formulation HD-03/ES capsules in the management of patients with chronic hepatitis B infection.METHODS: A tot

期刊

HD-03/ESChronic hepatitis BLiver function testsHepatitis B Virus markersClin

Role of bacteria in the etiopathogenesis of inflammatory bowel disease

Increased numbers of mucosa-associated Escherichia coli are observed in both of the major inflammatory bowel diseases,Crohn’s disease(CD)and ulcerative colitis

期刊

Adherent-invasive Escherichia coliCrohn's diseaseInflammatory bowel disea

Carotid artery stenting in patients with coexistent carotid and coronary artery disease

Atherosclerotic disease, as a systemic process, affects all arteries to varying degrees. In particular,coexistent carotid and coronary artery diseases are commo

期刊

carotid artery stentingcarotid artery diseasecoronary artery disease

Small hepatocellular carcinoma with peripheral enhancement:pathological correlation with dual phase

Background The peripheral enhancement of small hepatocellular carcinoma (SHCC) is a rare appearance in dual phase images by helical computed tomography (CT). Th

期刊

carcinomahepatocellulartomographyX-ray computedperipheral enhancement

Combined monitoring of evoked potentials during microsurgery for lesions adjacent to the brainstem a

Background Neurophysiologic monitoring during surgery is to prevent permanent neurological injury resulting from surgical manipulation. To improve the accuracy

期刊

motor evoked potentialtranscranial electrical stimulationsomatosensory evoked

中国柱隔孢属的分类研究Ⅷ.

山莴苣Mulgedium sp.和紫苜蓿Medicago sativa Linn.上两个柱隔孢属中国新记录种Ramularia mulgedii和R. medicaginis,另外三个已知种的名称作了订正.

期刊

山莴苣柱隔孢苜蓿柱隔孢中国新记录种

A new cucurbitane triterpenoid from Momordica charantia

One new cucurbitane-type triterpenoid saponin, 5β,19-epoxycucurbita-6,23-diene-3β,19,25-triol-3-O-β-D-allopyranoside (1),named momordicoside P was isolated f

期刊

Momordica charantiaCucurbitane triterpenoidSaponinMomordicoside P

泛函连接网络计算软件及其在生物多样性研究中的应用

针对农田生物多样性分析的需要,研制出泛函连接网络(FLANN)计算软件.该软件由7个Java类和1个HTML文件组成,是一种Internet在线计算工具,可运行于多种操作系统和Web浏览器上,

期刊

泛函连接网络计算软件Internet农田生物多样性

西藏冬虫夏草无性型的分子生物学研究

从西藏采集的冬虫夏草子实体中分离出 3个菌株C3、C4、C5,通过培养特征的研究以及采用分子生物学方法 ,以rDNAITS区为分子指标 ,与冬虫夏草 [Cordycepssinensis(Berk .)Sacc.

期刊

冬虫夏草中国被毛孢无性型rDNAITS区西藏

重组大肠杆菌热稳定性过氧化氢酶的纯化及性质研究

与本文相关的学术论文