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为了观察丹参酮ⅡA对腹腔注射腹膜透析液(PDS)大鼠腹膜形态以及全身和腹腔局部氧化应激反应的影响,将40只SD雄性大鼠随机分为4组:对照组,每日腹腔注射生理盐水20mL;PDS组,每日腹腔注射4.25%PDS20mL;丹参酮低、高浓度组,每日分别腹腔注射含丹参酮ⅡA浓度为50mg/L、100mg/L的4.25%PDS20mL。于实验第30天,取壁层腹膜行光镜检查,并检测血清和透出液中MDA、SOD、GSH浓度。结果表明HE、Masson染色显示,在PDS干预下,腹膜厚度显著增厚,胶原沉积显著增多,血清及透出液中MDA含量显著升高,SOD水平和GSH水平显著降低。与PDS组比较,用丹参酮的两组腹膜厚度减少,MDA水平显著降低,SOD水平和GSH水平都显著升高。丹参酮IIA具有潜在抑制葡萄糖PDS导致的实验大鼠腹膜纤维化及氧化应激的作用。
In order to observe the effect of Tanshinone ⅡA on peritoneal morphology and systemic and peritoneal local oxidative stress in peritoneal dialysis rats, 40 SD male rats were randomly divided into 4 groups: control group, intraperitoneal injection of physiology Saline 20mL; PDS group, intraperitoneal injection of 4.25% PDS 20mL daily; tanshinone low and high concentration group, intraperitoneal injection of tanshinone IIA concentration 50mg / L, 100mg / L 4.25% PDS 20mL daily. On the 30th day of the experiment, the parietal peritoneum was examined by light microscopy, and the concentrations of MDA, SOD and GSH in serum and effusion were measured. The results showed that HE and Masson staining showed that under the intervention of PDS, the peritoneal thickness was significantly increased, collagen deposition was significantly increased, MDA content in serum and permeate significantly increased, SOD and GSH levels significantly decreased. Compared with PDS group, the peritoneal thickness of tanshinone group decreased, MDA level decreased significantly, SOD level and GSH level increased significantly. Tanshinone IIA has the potential to inhibit peritoneal fibrosis and oxidative stress induced by glucose PDS in rats.