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从新生小牛肝及大鼠肝大部切除后的肝中用酒精反复沉淀和超滤方法提取肝生长刺激因子(HSS),利用32%肝部分切除后30小时的大鼠为检测模型研究HSS对大鼠再生肝细胞增殖的影响,结果发现,牛和鼠HSS均可使再生肝细胞分裂指数提高2～3倍,提示HSS可以促进肝细胞的分裂。牛HGS的剂量增加,其促肝细胞分裂的作用也随之增强。采用超滤法证明,HSS活性成分的分子量低于50KD。
Hepatic growth stimulating factor (HSS) was extracted from newly born calf liver and rat liver after partial resection by repeated alcohol precipitation and ultrafiltration. HSS was studied using a rat model of 32 hours after partial hepatectomy On the proliferation of rat regenerated hepatocytes. The results showed that HSS can increase the division index of regenerative hepatocytes by 2-3 times, suggesting that HSS can promote the division of hepatocytes. The dose of bovine HGS increased, and its role in promoting liver cell division also increased. Using ultrafiltration method to prove that HSS active ingredient molecular weight below 50KD.