本文以山麦冬水溶提取物为诱导分化剂,观察其对HL_(60)组胞的诱导分化作用,发现HL_(60)细胞在山麦冬作用下,细胞形态变得不规则,细胞核呈杆状或分叶状,核仁消失,胞质出现特殊颗粒,NBT阳性细胞率明显升高,c-myc原癌基因表达下降。上述结果表明,山麦冬具有明显的促分化作用,值得进一步研究。 In this study, a water soluble extract of Ophiopogon japonicus was used as a differentiation inducer to observe its effect on the induction and differentiation of HL_(60) cells. It was found that HL_(60) cells became irregular under the action of Ophiopogon japonicus, and the nucleus became a rod. Like or lobulated, the nucleolus disappeared, and special particles appeared in the cytoplasm. The rate of NBT positive cells increased significantly and the expression of c-myc proto-oncogene decreased. The above results show that Ophiopogon japonicus has obvious pro-differentiation effects and is worthy of further study.