利用ＮＡＡ、２，４－Ｄ、ＩＡＡ、ＩＢＡ、６－ＢＡ５种生长调节剂的不同浓度和不同组合配比，完成了对毛白杨叶愈伤组织诱导和完整植株的再生。在６－ＢＡ０．５ｍｇ／Ｌ分别和ＮＡＡ０．２～２．０ｍｇ／Ｌ、２，４－Ｄ２．０ｍｇ／Ｌ、ＩＡＡ２．０ｍｇ／Ｌ共同作用下，叶愈伤组织诱导率均能达到１００％，愈伤组织在６－ＢＡ１．０ｍｇ／Ｌ和ＮＡＡ０．２ｍｇ／Ｌ作用下芽诱导率为８５％，而适当浓度的ＮＡＡ和ＩＢＡ有促进试管苗生根的作用 The callus induction and whole plant regeneration of Populus tomentosa leaf were completed by using different concentrations of NAA, 2,4-D, IAA, IBA and 6-BA growth regulators and different combinations. Under the combination of 6-BA 0.5 mg / L and NAA 0.2-2.0 mg / L, 2,4-D2.0 mg / L and IAA 2.0 mg / L respectively, the callus induction rate reached 100% , The callus induction rate was 85% under the effect of 6-BA1.0mg / L and NAA0.2mg / L, while NAA and IBA of proper concentration could promote the rooting of plantlets