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目的:探讨荧光定量PCR技术(FQ-PCR)在婴幼儿沙眼衣原体肺炎早期诊断中的应用。方法:将2007年1月~2009年4月份收治的986例0~3岁婴幼儿肺炎作为研究对象,根据年龄分组,对各组婴幼儿均通过咽拭子获取标本,进行沙眼衣原体DNA(CT-DNA)测定;总结分析CT-DNA阳性病例。结果:986例标本中,CT-DNA阳性64例;其中3个月以内婴儿CT肺炎检出率最高,达13.8%,CT肺炎往往为无发热性肺炎,常伴有结膜炎是其特征,而X线多表现为支气管肺炎,其临床表现无特异性。结论:CT是婴幼儿肺炎的病原体之一;CT肺炎在婴幼儿3个月内检出率最高;FQ-PCR法是检测CT-DNA最直接、可靠的方法。
Objective: To investigate the application of fluorescence quantitative PCR (FQ-PCR) in the early diagnosis of Chlamydia trachomatis pneumonia in infants. Methods: A total of 986 infants aged 0-3 years with pneumonia admitted from January 2007 to April 2009 were enrolled in this study. According to the age groups, all infants in each group were taken throat swab to obtain the specimens of C. trachomatis DNA -DNA) determination; summary analysis of CT-DNA positive cases. Results: Of the 986 specimens, CT-DNA was positive in 64 cases. Among them, the detection rate of CT pneumonia in infants was the highest within 13 months (up to 13.8%). CT pneumonia was often characterized by non-febrile pneumonia, which was often accompanied by conjunctivitis More X-ray showed bronchial pneumonia, the clinical manifestations of non-specific. Conclusion: CT is one of the pathogens of pneumonia in infants and young children. CT pneumonia has the highest detection rate in infants and young children within 3 months. FQ-PCR method is the most direct and reliable method to detect CT-DNA.