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Kr1基因是小麦远缘杂交不亲和主效基因,为了解其表达及调控机制,本研究系统比较了小麦自花授粉和小麦×玉米远缘杂交两个过程中Kr1基因的表达差异,同时分析了这两个过程中Kr1基因部分序列的DNA甲基化状态。结果表明,Kr1基因在小麦自花授粉过程中始终处于低量表达或不表达状态,而在小麦×玉米远缘杂交过程中的表达呈动态变化,具体表现为授粉前低量表达,授粉后迅速大量表达,24h后又恢复为低量表达,高峰表达时期在外源花粉授入后0.5~2h左右。DNA甲基化分析显示,小麦自花授粉前后Kr1基因一直处于较高水平的甲基化状态,分别为58%和62%;而在授以玉米花粉后,Kr1基因迅速去甲基化,在0.5h内降到12%,在其后的1、2、24h内一直维持在10%~12%的低甲基化水平,表明DNA甲基化修饰参与了Kr1基因在小麦×玉米远缘杂交中的表达调控。
In order to understand the mechanism of its expression and regulation, Kr1 gene was a major incompatible major gene in wheat distant crosses. In this study, we systematically compared the expression of Kr1 gene between wheat self-pollination and wheat × maize distant hybridization The DNA methylation status of partial sequences of the Kr1 gene in these two processes. The results showed that the expression of Kr1 gene was always under low or no expression in the process of wheat self-pollination. However, the expression of Kr1 gene in the wheat × maize distant hybridization showed a dynamic change, which was characterized by low expression before pollination, After 24 hours, it returned to a low level. During the period of peak expression, it was about 0.5 ~ 2 hours after the pollen was introduced. DNA methylation analysis showed that the Kr1 gene was at a high level of methylation status before and after self-pollination, which were 58% and 62%, respectively. However, Kr1 gene was demethylated immediately after pollination, It decreased to 12% in 0.5h and remained at hypomethylation level of 10% ~ 12% in 1, 2 and 24 h, indicating that DNA methylation modification involved in the Kr1 gene hybridization in wheat × maize In the regulation of expression.