本研究试验地位于中国农业大学曲周试验站,于2002年3月开始按照有机、绿色和常规3种不同生产模式进行温室蔬菜长期试验研究。本试验分别于2009年1月18日、4月5日、7月11日、10月11日4次,分别在0～20cm和20～40 cm土层按S取样法取样,采用常规平板培养和菌落计数方法,测定土壤微生物的数量,旨在揭示不同管理方式土壤微生物数量和动态变化,为有机农业生产中的土壤管理提供依据。研究结果表明:有机管理方式明显促进土壤细菌数量的增长。其中,在0～20 cm土层中4～10月细菌数量变化为有机处理>常规对照,且在4月份和10月份达到显著水平;在20～40 cm土层:细菌的数量表现为有机处理>绿色处理、有机处理>常规对照,4～10月份有机处理细菌数量与常规对照有显著差异。有机管理方式明显促进土壤放线菌数量的增长。在0～20 cm和20～40 cm土层中,4～10月份放线菌数量变化为有机处理>绿色处理>常规对照,在0～20 cm土层,有机处理和常规对照之间有显著性差异;20～40 cm土层,10月份有机处理和常规对照达到显著水平。有机管理方式明显减少土壤真菌的数量。在0～20 cm土层,真菌数量变化为有机处理<绿色处理<常规对照,10月份有机处理和常规对照达到显著性水平;20～40 cm土层真菌数量变化趋势与0～20 cm土层真菌的数量变化趋势相反。 This experiment was located in Quzhou Experimental Station of China Agricultural University. In March 2002, long-term experiment on greenhouse vegetables was conducted according to three different organic, green and conventional modes of production. The experiment was carried out on January 18, April 5, July 11 and October 11, 2009 respectively, and samples were taken at 0-20 cm and 20-40 cm soil layers by S sampling method. And colony counting method to determine the quantity of soil microorganisms. The aim is to reveal the quantity and dynamic changes of soil microorganisms in different management modes, and provide the basis for soil management in organic farming. The results show that: organic management significantly promote the growth of soil bacteria. Among them, the number of bacteria changed from April to October in 0 ~ 20 cm soil layer to organic treatment> routine control, reaching significant level in April and October; in 20 ~ 40 cm soil layer, the number of bacteria showed organic treatment > Green treatment, organic treatment> conventional control, 4 to October the number of organic bacteria treatment significantly different from the conventional control. Organic management significantly promoted the increase of soil actinomycetes. The number of actinomycetes changed from April to October in 0 ~ 20 cm and 20 ~ 40 cm soil layers in the order of organic treatment> green treatment> normal control. There was a significant difference between 0 ~ 20 cm soil layer and 0 ~ 20 cm soil layer In 20-40 cm soil layer, organic treatment and conventional control reached the significant level in October. Organic management significantly reduces the amount of soil fungus. In 0 ~ 20 cm soil layer, the number of fungi changed to organic treatment