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目的建立了液相色谱-串联质谱测定婴幼儿食品中6种黄曲霉毒素的方法。方法试样经乙腈超声提取,离心,MycosepTM226净化柱净化后液相色谱串联质谱仪直接测定,外标法定量。结果加标回收率范围为83.3%~100.6%,相对标准偏差(RSDs)在1.30%~6.64%。将本方法用于实际样品分析,60份婴幼儿食品中黄曲霉毒素B1检出2份,黄曲霉毒素G2检出1份,其余4种黄曲霉毒素均未检出。结论此方法简便快速,可用于婴幼儿食品中6种黄曲霉毒素的测定。
Objective To establish a method for the determination of six aflatoxins in infant food by liquid chromatography - tandem mass spectrometry. Methods The samples were extracted by acetonitrile and centrifuged. The samples were purified by liquid chromatography tandem mass spectrometry after MycosepTM226 purification column was cleaned up and quantified by external standard method. Results The recoveries ranged from 83.3% to 100.6% with relative standard deviations (RSDs) ranging from 1.30% to 6.64%. The method was applied to the actual sample analysis. Aflatoxin B1 was detected in 60 samples of infants and young children, 1 in aflatoxin G2 and 1 in the remaining 4 aflatoxins. Conclusion This method is simple and rapid and can be used for the determination of six aflatoxins in infant food.