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目的:采用气相色谱-质谱联用(GC-MS)技术分离鉴定藏药“渣驯”中的挥发性成分和脂溶性成分。方法:水蒸气蒸馏法提取渣驯的挥发性成分,用石油醚提取渣驯的脂溶性成分,用GC-MS方法分离鉴定,应用峰面积归一化法计算各个化合物相对含量。结果:从渣驯挥发油中共鉴定出化合物41种,占总量的45.67%,相对含量在2%以上的成分有6种:泪杉醇(11.97%),马鞭烯醇(6.38%),α-可巴烯(2.67%),(E)-2-十四烯(2.56%),柏木脑(2.49),二十二烷(2.17%);从渣驯脂溶性提取物中共鉴定出化合物45种,占总量的92.86%,相对含量在2%以上的有14种:二十烷(12.83%),二十五烷(11.60%),棕榈酸甲酯(8.88%),三十烷(6.82%),十七烷(5.94%),(E)-5-二十碳烯(5.92%),1-二十六烷醇(3.50%),三十一烷(3.43%),泪杉醇(3.18%),二十酸甲酯(3.15%),十八酸甲酯(2.94%),二十二烷酸甲酯(2.89%),二十一烷(2.55%),二十四酸甲酯(2.15%)。结论:对渣驯挥发性与脂溶性成分进行了研究,为进一步明确渣驯的形成机制提供研究基础。
OBJECTIVE: To isolate and identify the volatile components and fat-soluble components of Tibetan medicine residue by using gas chromatography-mass spectrometry (GC-MS). Methods: Volatile components were extracted by steam distillation. The fat-soluble components were extracted by petroleum ether and identified by GC-MS. The relative content of each compound was calculated by the peak area normalization method. Results: A total of 41 compounds (45.67% of the total compounds) were identified from the volatile oil of tame tame oil. There were 6 compounds with the relative content of 2% or more: salbutamol (11.97%), whipol (6.38% Catechin (2.67%), (E) -2-tetradecene (2.56%), cypress wood (2.49) and docosane (2.17% , Accounting for 92.86% of the total. There were 14 kinds of eicosane (12.83%), pentacosane (11.60%), methyl palmitate (8.88%), (5.94%), (E) -5-eicosene (5.92%), 1-hexacosanol (3.50%), triacontane (3.43%), (3.18%), methyl erucate (3.15%), methyl octadecanoate (2.94%), methyl behenate (2.89%), eicosane (2.55%), Methyl ester (2.15%). Conclusion: The research on the volatile and fat-soluble components of tame grazing has provided the research basis for further clarifying the formation mechanism of dregs.