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目的:分析石榴花挥发油的化学成分,并测定其体外抗氧化活性。方法:采用水蒸气蒸馏法提取石榴花中的挥发油,用GC-MS对其进行分析;以对金属离子螯合作用和ABTS自由基清除作用为指标评价石榴花挥发油的抗氧化活性。结果:从石榴花挥发油中分离鉴定23个化合物,占挥发油总量的88.49%;20.00%石榴花挥发油对金属离子的螯合作用和ABTS自由基的清除作用优于0.10 mg·mL-1的EDTA或VC。结论:石榴花挥发油中含有多种有应用价值的化学成分,具有显著的体外抗氧化活性。
OBJECTIVE: To analyze the chemical constituents of volatile oil from pomegranate flower and determine its antioxidant activity in vitro. Methods: Volatile oil from pomegranate flower was extracted by steam distillation and analyzed by GC-MS. The antioxidant activity of volatile oil from pomegranate flower was evaluated by metal ion chelation and ABTS free radical scavenging. Results: 23 compounds were isolated and identified from volatile oil of pomegranate flower, accounting for 88.49% of the total volatile oil. The chelating effect of 20.00% volatile oil on pomegranate and ABTS radical scavenging activity were better than that of 0.10 mg · mL-1 EDTA Or VC. Conclusion: The volatile oil of pomegranate contains a variety of valuable chemical components, with significant antioxidant activity in vitro.