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目的:研究千佛菌对1型糖尿病模型小鼠细胞因子基因异常表达的调节作用及血糖水平、小鼠行为变化的影响,以探讨千佛菌对1型糖尿病小鼠模型的双向免疫调节作用。方法:观察千佛菌大、小剂量组、模型对照组与正常对照组小鼠行为变化;血糖的水平;淋巴细胞IL-2m RNA、IL-10m RNA的表达水平。结果:模型对照组小鼠表现为“三多一少”的症状:饮食多,饮水多,尿量增多,体型瘦弱。千佛菌大、小剂量组症状较模型对照组有所减轻,而正常对照组未见异常。与正常对照组比较,模型对照组血糖水平、IL-2m RNA表达水平明显增高(P<0.01),而IL-10m RNA表达水平明显降低(P<0.01);与模型对照组比较,千佛菌大、小剂量组的血糖水平、IL-2m RNA表达水平均明显降低(P<0.01、P<0.05),IL-10m RNA表达水平均明显增高(P<0.01、P<0.05);而千佛菌大、小剂量组的血糖水平、IL-2m RNA及IL-10m RNA表达水平的差异无统计学意义(P>0.05)。结论:千佛菌具有降低1型糖尿病模型小鼠血糖的作用,能下调IL-2m RNA的表达,上调IL-10m RNA的表达,对1型糖尿病模型小鼠脾脏淋巴细胞Thl/Th2细胞因子的平衡具有调节作用。
Objective: To study the regulatory effect of Thousand Flavus on the abnormal expression of cytokines in mice with type 1 diabetes mellitus and the effects of blood glucose level and behavioral changes in mice to explore the bidirectional immunomodulatory effects of Thousand Flavus on type 1 diabetic mice. Methods: The behavioral changes of mice in large and small dose group, model control group and normal control group were observed; the level of blood glucose and the expression of IL-2m RNA and IL-10m RNA in lymphocytes were observed. Results: The mice in the model control group showed symptoms of “more than one, less”. They had more diet, more drinking water, more urine, and thinner body. Thousands of bacteria, large dose group symptoms than the model control group has been alleviated, while the normal control group no abnormalities. Compared with the normal control group, the levels of blood glucose and IL-2m RNA in the model control group were significantly increased (P <0.01), while the levels of IL-10 mRNA were significantly decreased (P <0.01). Compared with the model control group, (P <0.01, P <0.05), and the levels of IL-10 mRNA in the large and small dose groups were significantly increased (P <0.01, P <0.05) There were no significant differences in blood glucose level, IL-2m RNA and IL-10m RNA expression between the large and small dose groups (P> 0.05). Conclusions: Thunbergii can decrease the blood glucose of type 1 diabetic mice, down-regulate the expression of IL-2m RNA and up-regulate the expression of IL-10mRNA in mice with type 1 diabetes. Balance has a regulatory role.