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本实验以白色念珠菌提取物用NIH小鼠做了外周血淋巴细胞微核试验,结果实验组的微核出现率比对照组显著增高(P<0.01);同时用高效液相色谱—荧光分光光度法对白色念珠菌提取物做了霉菌毒素分析,结果表明乙醚提取物,三氯甲烷提取物、水溶性成份均含黄曲霉毒素G_1,其量依次为2.08、2.44、0.07μg/kg,水溶性成份中还含黄曲霉毒素B_21.25μg/kg。毒素总量为5.84μg/kg。因此我们认为白色念珠菌可产生致突变物和致癌的霉菌毒素,为白色念珠菌致癌提供了实验依据。
In this experiment, peripheral blood lymphocyte micronucleus test was performed with NIH mice using Candida albicans extracts. As a result, the occurrence rate of micronucleus in the experimental group was significantly higher than that in the control group (P<0.01). Simultaneous HPLC-fluorescence spectrometry was used. Photometric analysis of the mycotoxins of Candida albicans extracts showed that the ether extract, trichloromethane extract, and water-soluble components all contained aflatoxin G1, which in turn was 2.08, 2.44, and 0.07 μg/kg. The ingredients also contain aflatoxin B_2 1.25μg/kg. The total amount of toxin was 5.84 μg/kg. Therefore, we believe that Candida albicans can produce mutagens and carcinogenic mycotoxins, providing experimental basis for Candida albicans cancer.