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DTPA法制备了 Eu3+ 标单抗 ,采用夹心法建立了 AFP和 CEA IFMA。它们的灵敏度分别为 43 ng/L和 90 ng/L ;批内CV为 1.5 99%和 1.3 6% ;批间 CV为 7.13 9%和 2 .89% ;平均回收率为 10 3 .98%和 92 .78%。与 CL IA进行临床应用比较显示结果高度相关 ( AFP r=0 .90 40 ,CEA r=0 .970 5 )
Eu3+ monoclonal antibody was prepared by DTPA method and AFP and CEA IFMA were established by sandwich method. Their sensitivities were 43 ng/L and 90 ng/L, respectively; intra-lot CVs were 1.5 99% and 1.3 6%; inter-lot CVs were 7.13 9% and 2.89%; the average recovery was 103.98% and 92.78%. Comparison of clinical applications with CL IA showed highly correlated (AFP r=0.90 40, CEA r=0.970 5)