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应用放射配体结合实验方法,检定了人妊娠子宫峡部平滑肌毒蕈碱样胆碱受体(M受体)及亚型。结果表明:在人子宫峡部存在两种M受体亚型。并应用竞争性蛋白结合分析法,测定了ACh对人妊娠子宫峡部平滑肌细胞合成IP3的影响。
Radioligand binding assay was used to detect the muscarinic isoform of cholinergic receptor (M receptor) and its subtype in human uterine isthmus. The results showed that there are two M receptor subtypes in isthmus of human uterus. The effect of ACh on the synthesis of IP3 in human islet uterine isthmus cells was measured by competitive protein binding assay.