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目的:应用蛋白质芯片技术寻找前列腺癌标本中的特异性标志蛋白。方法:利用表面增强激光解吸离子化蛋白质质谱分析(Surface-enhancedlaserdesorption/ionization,SELDI)技术,分析22例前列腺癌根治术切除标本中癌组织特有的蛋白质表达谱型。结果:在前列腺癌组织中发现了一个分子量在24,782.56±107.27m/Z的蛋白质,我们暂称其为Pca-24蛋白,此蛋白在17例前列腺癌中16例表达为阳性(94%),在相应前列腺正常组织中无表达;在12例良性前列腺增生中亦无表达。应用激光捕获微解剖挑取肿瘤细胞,我们进一步证实Pca-24蛋白来源于前列腺癌细胞。结论:蛋白质芯片技术对于在组织标本中发现肿瘤标志蛋白是一种有效的工具;Pca-24很可能是一个有用的前列腺癌标志物。
OBJECTIVE: To identify specific marker proteins in prostate cancer samples by using protein chip technology. Methods: Surface-enhanced laser desorption / ionization (SELDI) technique was used to analyze the protein profiles of 22 cancerous tissues in radical resection of prostate cancer. RESULTS: A protein with a molecular weight of 24,782.56 ± 107.27 m / Z was found in prostate cancer tissue. We tentatively called it Pca-24 protein, which was expressed as positive in 16 of 17 prostate cancers (94%). The corresponding normal prostate tissue no expression; no expression in 12 cases of benign prostatic hyperplasia. Micro-dissection of tumor cells using laser capture revealed that Pca-24 protein was derived from prostate cancer cells. CONCLUSIONS: Protein chip technology is an effective tool for the discovery of tumor marker proteins in tissue specimens; Pca-24 is likely to be a useful marker of prostate cancer.