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目的研究乙型肝炎病毒(HBV)在新疆地区柯尔克孜族人群中的基因型分布情况,探讨柯尔克孜族人群中HBV基因型与C区变异相关性。方法 1.收集新疆地区柯尔克孜族HBV感染者血清,提取病毒DNA,应用荧光分型PCR(GQPCR)法与二代测序(NGS)技术进行HBV基因型分型检测,同时以实时荧光定量PCR法对其HBV DNA载量进行定量检测。2.通过生物信息学手段将HBV C区的测序结果与Gene Bank中的HBV参考株进行核苷酸同源性比对,确定HBV C区的变异规律。结果 1.HBV基因分型结果:111例柯尔克孜族样本成功分型99例,其中B型10例,占10.10%;C型6例,占6.06%;D型75例,占75.76%;混合型8例,占8.08%。柯尔克孜族HBV人群以D型为优势型。2.HBV-DNA定量检测结果显示:柯尔克孜族HBV-DNA载量在3~10 log10 copies/ml之间,平均5.67±1.04 log10 copies/ml。高、中、低拷贝组在柯族人群中的分布有显著差异。3.基因变异分析结果显示:不同基因型突变位点不同、变异频率差异显著。柯尔克孜族B、C型变异相似,D型中变异主要为G1896A,3种基因型都未发生G1862A变异。结论新疆地区柯尔克孜族群体乙型肝炎病毒的基因型分布具有本民族的自身特点,其各基因型与C区变异的相关性也具有显著差异,研究结果提示在新疆多民族地区进行乙肝防治时,应该根据不同民族群体HBV所具有的基因型和基因变异特点与规律,采取不同的防治策略,提高新疆各族人民的健康水平和卫生质量。
Objective To study the genotype distribution of Hepatitis B virus (HBV) in the Kirgiz population in Xinjiang and to explore the correlation between HBV genotype and mutation in C region in Kirgiz population. Methods 1. Serum of Kirgizi patients infected with HBV in Xinjiang were collected to extract the virus DNA. Genotyping of HBV genotypes was detected by GQPCR and NGS. Real-time fluorescence quantitative PCR Its HBV DNA load was quantified. Bioinformatics methods were used to compare the sequencing results of HBV C region with the HBV reference strain in Gene Bank to determine the variation of HBV C region. RESULTS: The genotyping results of HBV: 111 cases of Kirgiz were successfully classified into 99 cases, of which 10 cases were type B (10.10%), 6 cases were type C (6.06%), 75 cases (75.76%) were D type, 8 cases, accounting for 8.08%. Kirgiz HBV population to D-type predominance. HBV-DNA quantitative test results showed that: Kirgiz HBV-DNA load between 3 ~ 10 log10 copies / ml, an average of 5.67 ± 1.04 log10 copies / ml. There were significant differences in the distribution of high, medium and low copy groups in the Ko tribe population. Gene mutation analysis showed that: different genotypes of different mutation sites, the frequency of variation was significant. Kirchizi B, C type of variation is similar, D-type mutation is mainly G1896A, three genotypes did not occur G1862A mutation. Conclusion The genotype distribution of Hepatitis B virus in Kirgiz population in Xinjiang region has its own characteristics. The genotype distribution of Hepatitis B virus in Cik region is also significantly different from that in C region. The results suggest that in Hepatitis B prevention and treatment in Xinjiang, According to the characteristics and laws of HBV genotypes and genetic variations in different ethnic groups, different strategies should be taken to improve the health and quality of people of all nationalities in Xinjiang.