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本工作目的在于研究胆碱能受体阻断剂东莨菪碱(scopolamine)能否纠正大脑皮层癫痫放电.实验用大鼠31只,80万单位青霉素诱发急性全身性癫痫模型(n=19,ip),170单位青霉素直接施加在大鼠大脑皮层上诱发癫痫放电(n=12).全身性癫痫模型的主要放电形式有:阵发性、持续性多棘波连续发放,棘慢波综合,其放电振幅一般为180～800μV,侧脑室注射“莨菪化”剂量东莨菪碱4.5～12μg(n=14)明显压抑大脑皮层多棘波型癫痫放电振幅(10/14),也可以使多棘波型癫痫放电向高波幅慢波转化,以及减少异常放电指数.侧脑室注射相同剂量生理盐水对癫痫放电无明显影响(n=5).大脑皮层局部用青霉素主要诱发高波幅尖波型癫痫放电,腹腔注射东茛菪碱0.3mg/kg 明显降低癫痫放电频率,但对其振幅的影响较弱.结果提示:东莨菪碱能够压抑大脑皮层癫痫放电,但不能完全纠正之.
The aim of this work was to investigate whether scopolamine, a cholinergic receptor blocker, can correct cerebral cortical epileptic discharge. Experimental rats were used to induce acute systemic epilepsy (n=19, ip) in a total of 31 rats and 800,000 units of penicillin. A unit of 170 units of penicillin was applied directly to the rat cerebral cortex to induce epileptic discharge (n=12). The main forms of discharge in the systemic epilepsy model were: paroxysmal, persistent multiple spike-wave continuous firing, spike-slow-wave synthesis, and its firing amplitude. Normally 180-800μV, lateral cerebral ventricle injection of “sulfhydration” dose scopolamine 4.5 ~ 12μg (n = 14) significantly suppressed the cerebral cortical multiple spike-like epileptic discharge amplitude (10/14), but also can make multiple spike-like epilepsy discharge to High-amplitude slow-wave conversion and reduction of abnormal discharge index. Intraventricular injection of saline at the same dose had no significant effect on epileptic discharge (n=5). Topical cerebral cortex was predominantly induced by high-amplitude apical-wave type epileptic discharge with penicillin, and intraperitoneal injection of E. coli was performed. Caesarean 0.3mg/kg significantly reduced the frequency of epileptic discharge, but its effect on the amplitude was weak. The results suggest that scopolamine can suppress the cerebral cortex epileptic discharge, but can not be completely corrected.