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目的 :建立急性低氧状态下肺损伤动物模型 ,探讨高原西氏胶囊 (西氏胶囊 )对急性肺损伤的治疗作用及其机理。方法 :实验用雄性Wistar大鼠 ,放入小动物低压仓内 ,匀速减压至模拟海拔 60 0 0m高度气压 ,持续减压 1 2h。然后用油酸复制肺损伤模型 ,并观察西氏胶囊对肺损伤的防治作用。结果 :由上腔静脉注入油酸后 ,成功复制大鼠急性肺损伤模型 ,与注油酸前比较 ,呼吸频率增加明显 ,肺水含量 (湿 /干比值 )随时间的延长而加重。西氏胶囊组肺水含量显著降低 ,肺水肿和炎细胞浸润的病理变化均较油酸组轻。结论 :从插管的上腔静脉直接注入油酸复制急性肺损伤模型 ,成功率达 1 0 0 %。更接近于临床急性肺损伤的改变。西氏胶囊可使肺水肿减轻 ,炎细胞浸润减少 ,对急性低氧油酸肺损伤有较好的防治作用。
Objective : To establish an animal model of acute lung injury in acute hypoxia and to explore the therapeutic effect and mechanism of Xishi capsule on acute lung injury. METHODS : Male Wistar rats were used in the experiment and placed in the low pressure chamber of a small animal. The pressure was decompressed at a constant speed to a height of 6,000 m above sea level and decompression was continued for 12 hours. Lung injury model was then replicated with oleic acid and the effect of Xishi capsule on lung injury was observed. RESULTS: After injection of oleic acid into the superior vena cava, the rat model of acute lung injury was successfully replicated. Compared with before oleic acid injection, the respiratory rate increased significantly, and the lung water content (wet/dry ratio) increased with time. The lung water content in Xishi capsule group was significantly decreased, and the pathological changes of pulmonary edema and inflammatory cell infiltration were lighter than those in oleic acid group. Conclusion: The oleic acid replication acute lung injury model was injected directly from the superior vena cava of the intubation. The success rate was 100%. It is closer to the change of clinical acute lung injury. Xishi capsule can reduce the pulmonary edema and reduce the infiltration of inflammatory cells, and it has better prevention and treatment of acute hypoxia oleic acid lung injury.