论文部分内容阅读
目的研究上调婴幼儿血管瘤内皮细胞miR-206水平对细胞增殖、凋亡和侵袭能力的影响。方法通过CD31磁珠提取婴幼儿血管瘤内皮细胞,经转染miR-206模拟物提高HemECs细胞内miR-206水平,研究miR-206对该细胞功能的影响。以cck-8法检测细胞增殖能力的改变,AV-PI凋亡试剂盒经流式细胞仪检测凋亡水平变化,并经Transwell侵袭实验检测侵袭能力变化。结果转染miR-206模拟物进入HemECs细胞内48 h后,能够明显抑制细胞增殖,抑制率为(34.2±6.8)%;转染48 h后相比于对照组(1.7±1.6)%的凋亡细胞比率,miR-206模拟物组细胞凋亡比率提高到(23.9±5.1)%,差异具有统计学意义;转染后24 h侵袭实验显示,细胞穿透Transwell小室至下室面细胞数在对照组为30.3±2.4,在miR-206模拟物组细胞数减少至14.3±3.0。结论上调HemECs细胞内miR-206水平可明显抑制细胞增殖,增加细胞凋亡水平,减少细胞侵袭能力。提示miR-206可能通过调节瘤体细胞生物活性,对婴幼儿血管瘤疾病进程起到重要的调控作用。
Objective To investigate the effects of miR-206 on the proliferation, apoptosis and invasion of infantile hemangiomas. Methods The endothelial cells of infantile hemangiomas were isolated by CD31 magnetic beads. MiR-206 mimics were transfected into HemECs to increase the level of miR-206 in HemECs. The effects of miR-206 on the function of the cells were studied. The changes of cell proliferation were detected by cck-8 method. The apoptosis rate was detected by AV-PI apoptosis kit by flow cytometry, and the invasion ability was detected by Transwell invasion assay. Results After transfected into HemECs cells for 48 h, miR-206 mimics could significantly inhibit the proliferation of cells (34.2 ± 6.8%). Compared with the control group (1.7 ± 1.6)% The percentage of apoptotic cells in miR-206 mock group increased to (23.9 ± 5.1)%, and the difference was statistically significant. At 24 h after transfection, the number of apoptotic cells in Transwell chamber was The control group was 30.3 ± 2.4 and the number of cells in the miR-206 mock group was reduced to 14.3 ± 3.0. Conclusion Up-regulation of miR-206 level in HemECs can significantly inhibit cell proliferation, increase apoptosis and decrease cell invasiveness. It is suggested that miR-206 may play an important regulatory role in the process of infantile hemangiomas by regulating the biological activity of tumor cells.