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为了开发新型的药物缓释载体,以丝素纳米颗粒和β-环糊精为原料,以天然产物京尼平为交联剂,制备丝素纳米颗粒/β-环糊精菱形高分子微球。采用扫描电子显微镜(SEM)观察、红外光谱(FT-IR)分析、X射线衍射(XRD)分析、差示扫描量热(DSC)分析、热重(TG)分析等方法对制备的菱形高分子微球的结构和性能进行测试与表征。结果表明:制备的菱形高分子微球粒径在0.5~1.0μm之间,京尼平的添加使丝素纳米颗粒与β-环糊精发生融合,对菱形高分子微球的形成起到关键性作用;微球中丝素蛋白主要以β-折叠构象存在,丝素蛋白与京尼平交联后形成新的共价键,其结晶性能提高,热稳定性能增强。基于丝素纳米颗粒/β-环糊精菱形高分子微球的上述良好性能,以及原料组分自身的良好生物相容性和可降解性,预测其有望作为一种新型给药载体。
In order to develop a new drug sustained release carrier, silk fibroin nanoparticles / β-cyclodextrin rhombohedral polymer microspheres were prepared by using silk fibroin nanoparticles and β-cyclodextrin as raw materials and natural product genipin as a crosslinking agent . The prepared diamond polymer was characterized by scanning electron microscopy (SEM), infrared spectroscopy (FT-IR), X-ray diffraction (XRD), differential scanning calorimetry (DSC) and thermogravimetric analysis (TG) Microsphere structure and properties were tested and characterized. The results show that the diameter of rhombohedral polymer microspheres is between 0.5 and 1.0 μm, and the addition of genipin causes the fusion of fibroin nanoparticles with β-cyclodextrin, which is crucial to the formation of rhombohedral microspheres The silk fibroin in the microspheres mainly exists in the β-sheet conformation, and the new covalent bond is formed after the silk fibroin is cross-linked with the genipin. The crystallinity is improved and the thermal stability is enhanced. Based on the above-mentioned good properties of the silk fibroin nanoparticles / β-cyclodextrin rhombic polymer microspheres and the good biocompatibility and biodegradability of the raw material component itself, it is expected to be used as a novel drug delivery carrier.