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目的探讨胰岛素样生长因子（ＩＧＦｓ）多肽、受体和结合蛋白在免疫调节和免疫发育中的意义。方法采用逆转录聚合酶链反应技术在ｍＲＮＡ水平观察正常成人和新生儿淋巴细胞静息和ＰＨＡ刺激不同时间ＩＧＦｓ及其受体，结合蛋白基因的表达。结果在静息和活化的成人和新生儿淋巴细胞均有ＩＧＦ－Ⅰ、ＩＧＦ－ⅠＲ、ＩＧＦ－ⅡＲ、ＩＧＦＢＰ－２、３、４、６基因表达，并随ＰＨＡ刺激时间的延长表达水平有所变化；ＩＧＦＢＰ－５ｍＲＮＡ在活化的淋巴细胞表达；ＩＧＦ－Ⅱ、ＩＧＦＢＰ－ⅠｍＲＮＡ仅在新生儿淋巴细胞检测到。结论ＩＧＦｓ可能通过参与淋巴细胞的增殖、分化，影响免疫系统的功能和免疫发育过程。
Objective To investigate the significance of insulin-like growth factor (IGFs) polypeptides, receptors and binding proteins in immune regulation and immune development. Methods Reverse transcription polymerase chain reaction (RT-PCR) was used to observe the expression of IGFs and their receptor and binding protein genes in resting and PHA-stimulated normal adult and neonatal lymphocytes at different mRNA levels. Results The expressions of IGF-Ⅰ, IGF-ⅠR, IGF-ⅡR and IGFBP-2, 3, 4 and 6 mRNA in resting and neonatal lymphocytes were both higher than those in PHA group IGFBP-1mRNA was detected only in neonatal lymphocytes. Conclusion IGFs may affect the function of the immune system and the process of immune development by participating in the proliferation and differentiation of lymphocytes.