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目的探讨大肠癌 p16基因缺失与临床病理特征的关系。方法用聚合酶链反应技术 (PCR)对 3 2例大肠癌患者手术切除标本及粘膜正常组织进行 p16基因第 2外显子检测。结果 p16基因在癌组织中缺失率为 5 3 .1% ,与大肠粘膜正常组织的 6.3 %有显著性差异(P<0 .0 1) ,且与大肠癌病理组织学分型 ,临床分期及淋巴结转移情况有关。结论 p16基因缺失与大肠癌发生发展及分化不良有关。检测 p16基因缺失情况可为大肠癌临床诊治及预后判断提供较为可靠的依据
Objective To investigate the relationship between p16 gene deletion and clinicopathological features in colorectal cancer. Methods The exon 2 of p16 gene was detected by polymerase chain reaction (PCR) in 32 specimens of patients with colorectal cancer and normal mucosa. Results The deletion rate of p16 gene in cancer tissues was 53.1%, which was significantly different from that of 6.3% in colorectal mucosa tissues (P <0.01), and was significantly correlated with histological type, clinical stage and lymph node Transfer related. Conclusion The deletion of p16 gene is associated with the occurrence and differentiation of colorectal cancer. Detection of p16 gene deletion may provide a reliable basis for clinical diagnosis and prognosis of colorectal cancer