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目的:通过研究黄芪注射液对实验性胰腺纤维化大鼠氧化应激反应的影响,探索其缓解胰腺纤维化的机制。方法:大鼠随机分为6组,模型组和治疗组采用胰胆管注射2%TNBS溶液的方法制备慢性胰腺纤维化大鼠模型,治疗组分别给予低、中、高剂量的黄芪注射液治疗;造模后第6周,ELISA检测血清MDA含量和GSH-PX活性、免疫组化检测胰腺α-SMA和Ⅰ型胶原的含量。结果:与模型组相比,随着黄芪注射液的剂量的增加,除了GSH-PX活力逐渐恢复以外,治疗组大鼠血清中MDA含量逐渐降低且胰腺中α-SMA和Ⅰ型胶原含量逐渐较少,且均具有有统计学意义。结论:黄芪注射液可以减弱CP的氧化应激,抑制PSC活化,缓解胰腺的纤维化。
OBJECTIVE: To investigate the effect of Astragalus injection on oxidative stress in experimental rat with pancreatic fibrosis and to explore its mechanism of relieving pancreatic fibrosis. Methods: The rats were randomly divided into 6 groups. Chronic pancreatic fibrosis rat model was induced by injecting pancreaticobiliary into 2% TNBS solution in model group and treatment group. The treatment group was treated with low, medium and high doses of astragalus injection respectively. Six weeks after model establishment, serum MDA and GSH-PX activity were measured by ELISA. The contents of α-SMA and type Ⅰ collagen in pancreatic tissue were detected by immunohistochemistry. Results: Compared with the model group, with the increase of astragalus injection dose, the MDA content in the serum of rats in treatment group decreased gradually and the content of α-SMA and type Ⅰ collagen gradually increased Less, and have statistically significant. Conclusion: Astragalus injection can reduce the oxidative stress of CP, inhibit the activation of PSC and alleviate the fibrosis of the pancreas.