Objective:To construct a recombinant lentivirus vector which carries SD rat transducer of regulated CREB activity-1(TORC1) gene and examine its ability to express the TORC1 gene in vitro.Methods:The coding sequence of SD rat TORC1 gene was amplified using PCR and cloned into pGC-FU vector.293T cells were transfected using Lipofectamine 2000 and packaged for the recombinant lentivirus particles.When the cloned sequence was identified to be right,the recombinant lentivirus particles were amplified in a large quantity.The titer of virus was determined by real-time PCR and the level of TORC1 expression was examined by Western blot. Results:The recombinant lentivirus vector carrying TORC1 was constructed successfully and could express TORC1 at a high level in 293T cells in vitro,and the titer determined by real-time PCR was 2×108 TU/ml.Conclusion:The recombinant lentivirus vector could express TORC1 gene at a high level,and was very helpful in the study of exploring the effect of TORC1 on spinal cord injury. Objective: To construct a recombinant lentivirus vector which carries SD rat transducer of regulated CREB activity-1 (TORC1) gene and examine its ability to express the TORC1 gene in vitro. Methods: The coding sequence of SD rat TORC1 gene was amplified using PCR and cloned into pGC-FU vector.293T cells were transfected using Lipofectamine 2000 and packaged for the recombinant lentivirus particles. Now the cloned sequence was identified to be right, the recombinant lentivirus particles were amplified in a large quantity. The titer of virus was determined by Real-time PCR and the level of TORC1 expression was examined by Western blot. Results: The recombinant lentivirus vector carrying TORC1 was constructed successfully and could express TORC1 at a high level in 293T cells in vitro, and the titer determined by real-time PCR was 2 × 108 TU / ml. Conflusion: The recombinant lentivirus vector could express TORC1 gene at a high level, and was very helpful in the study of exploring the effect of TORC1 on spinal cord injury.