论文部分内容阅读
应用改良甲苯胺蓝染色法 (MTB )和阿尔辛蓝 番红鉴别染色法 (AB S )对静止期和活动期奶牛乳腺肥大细胞的粘膜免疫学性质进行了研究。MTB染色结果显示 ,静止期乳腺中的肥大细胞主要分布于各级输乳管上皮基膜区 ,小血管周围也有肥大细胞分布。在活动期 ,肥大细胞主要分布于腺泡上皮基膜附近 ,上皮内也有极少数肥大细胞分布 ,小叶间结缔组织中的肥大细胞则分布于小叶间导管或输乳管上皮基膜区。AB S染色证实 ,乳腺肥大细胞只被阿尔辛蓝染成蓝色 ,而不被结缔组织型肥大细胞的标志性染料番红着染。这表明 ,乳腺肥大细胞是一种典型的粘膜型肥大细胞 (MMC ) ,并参与构成乳腺粘膜免疫的第一道防线。
The mucosal immunological properties of mammary gland mast cells in quiescent and active period were studied by modified Toluidine blue staining (MTB) and Alcian blue-red staining (AB S). The results of MTB staining showed that the mast cells in quiescent mammary glands were mainly located in the basement membrane region of the ductus epithelialis and the distribution of mast cells around the small vessels. In the active phase, mast cells are mainly distributed in the vicinity of the basement membrane of the acinar epithelium, there are very few mast cells in the epithelium, and the mast cells in the interlobular connective tissue are distributed in the interlobular ducts or ducts. AB S staining confirmed that mast mast cells were only stained blue with Alcian blue and not stained with Safranin, the hallmark dye of connective tissue-type mast cells. This shows that mast mast cells are a type of typical mucosal mast cells (MMCs) and are involved in the first line of defense that forms the mucosal immunity of the breast.